Genotyping
This service is suitable for clients wanting to size PCR fragments for a number of different applications. Short Tandem Repeat (STR) analysis and Amplified Fragment Length Polymorphism (AFLP) analysis are just 2 examples of a wide variety of possible applications suitable for this service.
Scientific Services
Medvet can accommodate clients with either small batch numbers or large population screens. We encourage all prospective clients to discuss their needs with us so that any requirements are addressed on an individual basis.
Results are analysed using GeneScan and Genotyper software (from Applied Biosystems). If you do not have access to this software, we can send results as a hard copy to you by mail. Please contact us to discuss your requirements.
ABI PRISM® 3700 capillary DNA analyser is able to analyse fragments labelled with up to 4 dyes compatible with all Applied Biosystems dyes examples of dye combinations possible:
- 6-FAM™, HEX, NED™
- 6-FAM, VIC™, NED™, PET™
- 5-FAM™, JOE™, NED™
- dR110, dR6G, dTAMRA™, dROX™
- analysis of fragments with a size range of 50-500bp with a combination of up to 4 dyes
- analysis of fragments with a size range of 100-1000bp with a combination of up to 3 dyes
Size standard may be provided by the client (popular for clients screening many samples using 96 well plates on 3700 DNA analyser) or included in lane costs.
Sample Transport
Transport options (depending on client needs):
- 5-10ul PCR product in either separate 0.2mL tubes
- 0.2mL strip tubes
- 96 well plates
Samples should be marked for delivery to:
Medvet Genotyping Service
Division of Molecular Pathology
IMVS
Frome Road
Adelaide SA 5000
Delivery of results
If you are a registered client and have access to Genotyper® or GeneScan® software from Applied Biosystems we can arrange for you to receive your results using the internet.
Clients may choose to have electropherogram printouts of their samples mailed or faxed to them (a small administration fee applies).
Please contact us for assistance in planning dyes and fragment size ranges. Clients must be registered before sending samples for analysis.
With each batch of samples sent for analysis, please include a Genotyping Service Request Form.